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FAQ

Metadata

Should I provide tabular or ISA-Tab metadata?

Tabular metadata are simpler to prepare, while ISA-Tab has the added benefit of being able to note relationships between raw and processed files.

How do I create an ISA-Tab archive?

The simplest way is via this Google Sheet template, following these instructions:

  1. Fill out all 3 sheets
  2. Download each sheet as tab-separated values, naming the investigation, study, and assay sheets as i_<study_identifier>.txt, s_<study_identifier>.txt, and a_<study_identifier>.txt, respectively
  3. Compress the 3 .txt files as a .zip archive

Alternatively, if you prefer to have access to the full functionality of ISA-Tab, download the latest version of ISAcreator and follow along with this video tutorial to create an ISA-Tab archive.

Data Set Upload

How do I get my data into the Stem Cell Commons?

You have 3 options:

  • Option 1: Upload your data files alongside tabular metadata (e.g. txt, csv). Sample name, filename, and organism fields are required — the remaining fields are up to you.
  • Option 2: Upload your data files alongside 3-tier ISA-Tab metadata — the benefit of this option is that file relationships can be indicated. A Google Sheet-based ISA-Tab template is available here.
  • Option 3: Directly upload remote files via the AWS Command Line Interface. This option is available after providing either tabular or ISA-Tab metadata through the browser.

Collaboration

How do I share my data with another user?

As data set owner, you are able to share a data set, via the Data Sets card on My Dashboard, with a new or existing group. If the other user does not already belong to that group, invite him/her using the neighboring Collaboration card.

Harvard Medical School - Department of Biomedical Informatics - Harvard Chan Bioinformatics Core - Harvard Stem Cell Institute